Myo-Inositol Phosphates from Beef Brain Phosphoinositide

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Myo-inositol phosphates obtained by alkaline hydrolysis of beef brain phosphoinositide.

It is now apparent that the nutritional requirement for myoinositol in many cells is related to its participation as a building unit of myo-inositol-containing phospholipids (phosphoinositides). This explains why Eastcott (1)) in her classic work, found that all the myo-inositol taken up from the medium by growing yeast could be recovered by acid hydrolysis of the cells. The role of phosphoinos...

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Complete characterization of the myo-inositol polyphosphates from beef brain phosphoinositide.

The identification of a myo-inositol-containing phospholipid isolated from beef brain (1) as a triphosphoinositide has been reported in earlier publications from this laboratory (2) and in a note by Dittmer and Dawson (3). It has been established (2) by periodate oxidation that the myo-inositol moiety in the intact triphosphoinositide is substituted on the 1, 4, and 5 or 6 hydrosyl groups. When...

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The separation of myo-inositol phosphates by ion-pair chromatography.

The separation of myo-inositol phosphates by ion pair, reverse-phase high performance liquid chromatography has been investigated. The retention of the inositol phosphates is dependent on both the polarity of the hetaeron utilized and on the pH of the solvent. A method is presented which permits the isocratic separation of multiple forms of inositol phosphates including isomers of myo-inositol ...

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Nuclear magnetic resonance spectroscopic analysis of myo-inositol phosphates including inositol 1,3,4,5-tetrakisphosphate.

1H and 31P NMR spectra of a variety of phosphorylated myo-inositols have been analyzed using a Bruker WH-360 spectrometer. Proton and phosphorus chemical shifts and coupling constants are reported for myo-inositol 1-phosphate, myo-inositol 2-phosphate, myo-inositol 5-phosphate, myo-inositol 1,2-cyclic phosphate, myo-inositol 1,4-bisphosphate, myo-inositol 1,4,5-trisphosphate, and myo-inositol 1...

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Loss of murine Na+/myo-inositol cotransporter leads to brain myo-inositol depletion and central apnea.

myo-Inositol (Ins) and its polyphosphoinositide derivatives that are important in membrane signaling have long been held to play a special role in brain metabolism. As polyphosphoinositides turn over rapidly and are exceptionally abundant in nervous tissue, high Ins levels in the range of 2-15 mm that have been observed in brain may be necessary to maintain the rates of phosphoinositide synthes...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1960

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)76897-5